Table 2

Relative transcript levels* of repeats in ES cell lines

Minor satellite

Major satellite

X141


WT (OS25)

0.4 ± 0.1

1.8 ± 0.5

1.8 ± 0.3

Eed KO

7.2 ± 6.5

18.5 ± 17.0

1.8 ± 0.2

Dnmt1 KO

0.3 ± 0.1

2.1 ± 0.0

2.0 ± 1.3

Dnmt3a/3b DKO

0.1 ± 0.0

5.7 ± 2.4

1.5 ± 0.9

G9a WT

0.1 ± 0.0

0.7 ± 0.1

0.5 ± 0.7

G9a KO

0.4 ± 0.4

1.0 ± 0.2

ND

Suv39 h1/h2 WT

4.9 ± 0.8

2.5 ± 1. 6

1.1 ± 0.3

Suv39 h1/h2 DKO

4.0 ± 1.3

2.2 ± 0.7

2.1 ± 0.5

Dicer WT

0.2 ± 0.0

4.1 ± 0.0

2.7 ± 0.0

Dicer KO

0.9 ± 0.6

17.4 ± 5.5

4.1 ± 1.2

C2C12 dif

100

100

100


*Levels of repeat sequence RNA were normalized to a house keeping gene (Ube) and expressed as a percentage of the levels detected in differentiated mouse myocytes derived from C2C12 (C2C12 dif), samples in which high levels of major and minor satellite transcripts have previously been detected [56]. Controls without reverse transcriptase were analyzed in parallel to dismiss contamination with genomic DNA.

Four independent samples showed variable but increased transcript levels: major satellite, 8.2%, 8.7%, 13.4%, and 43.8% of C2C12; minor satellite, 2.6%, 7.1%, 11.8%, and 13.8% of C2C12. ND, none detected.

Jørgensen et al. Genome Biology 2007 8:R169   doi:10.1186/gb-2007-8-8-r169

Open Data