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Species-specific shifts in centromere sequence composition are coincident with breakpoint reuse in karyotypically divergent lineages

Kira V Bulazel1, Gianni C Ferreri1, Mark DB Eldridge3,2 and Rachel J O'Neill1*

Author Affiliations

1 Department of Molecular and Cell Biology, Mansfield Rd, University of Connecticut, Storrs, CT 06269, USA

2 Department of Biological Sciences, Macquarie University, NSW 2109, Australia

3 Molecular Biology, Australian Museum, College St, Sydney, NSW 2010, Australia

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Genome Biology 2007, 8:R170 doi:10.1186/gb-2007-8-8-r170

Published: 20 August 2007

Additional files

Additional data file 1:

All species names and corresponding accession numbers used in phylogenetic studies

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Additional data file 2:

For each species (left), probes used are indicated (top). For the pooled probe set, a combination of sat1 sequences from Mrob, Mpm, Wbi, Mrfs were used in one hybridization reaction. Hybridization time is indicated by the number (hyb #) of days probe is incubated at 37°C. The number of antibody detection layers is also indicated. All other conditions are described in the Materials and methods.

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Additional data file 3:

Probe images are in red and metaphase chromosomes are inverted DAPI

Format: EPS Size: 9.5MB Download file

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Additional data file 4:

Probe images are in red and metaphase chromosomes are inverted DAPI

Format: EPS Size: 9MB Download file

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Additional data file 5:

Probe images are in red and metaphase chromosomes are inverted DAPI

Format: EPS Size: 6.6MB Download file

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Additional data file 6:

Southern analyses of each satellite (sat1, B29 and sat23) to each species used in these analyses

Format: EPS Size: 6.9MB Download file

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Additional data file 7:

Cyt b nucleotide positions are numbered according to M. robustus numbering [GenBank:Y10524]; Cyt b spans 14,184 bp to 15,329 bp

Format: DOC Size: 55KB Download file

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