Figure 7.

Analysis of osteoblast, chondrocyte and myocyte specific markers in BMP2⁺ cells. (a) RT-PCR analysis in BMP2⁺ cells. (b,c) Alizarin stanings on the 18th day after plating the BMP2⁺ cells (7 + 18 days in total) and 28 days after plating (7 + 28, 35 days in total). (d) Relative expression levels of the genes presented in (a) as obtained by Affymetrix analysis. The expression levels of each gene were normalized with its maximum level set as 100%. Each result is an average of three independent experiments (Additional data file 13).