CS-CCC identifies protein interactions that could not be identified by CCC. (a) E. coli K12 cluster built around ArgT; (b) P. aeruginosa PA01 cluster built around ArgT; (c) an example of information revealed by CS-CCC but not by CCC. E. coli K12 proteins (green) that are co-conserved with E. coli ArgT (diamond) cluster were extracted. Then P. aeruginosa (mustard edge) and B. subtilis (red edge) proteins that are co-conserved with proteins in the E. coli ArgT cluster were extracted. Note that it is the B. subtilis network that shows a connection between amino acid biosynthesis proteins and flagellar proteins, via FliY (square). If only the E. coli cluster had been examined, as occurs using the CCC method, then this connection would have been missed.
Karimpour-Fard et al. Genome Biology 2007 8:R185 doi:10.1186/gb-2007-8-9-r185