Profiling strategy for myo-3::GFP muscle cells. Embryos are released from gravid adults and dissociated with chitinase. myo-3::green fluorescent protein (GFP) labeled muscle cells (green) were isolated by fluorescence-activated cell sorting (FACS) directly from freshly dissociated embryos to generate a profile of nascent body muscle cells (M0) and from embryonic cells after 24 hours in culture to obtain microarray data from fully differentiated muscle cells (M24). RNA extracted from each set of isolated muscle cells was amplified and labeled for hybridization to C. elegans whole genome Affymetrix arrays. GFP, green fluorescent protein.
Fox et al. Genome Biology 2007 8:R188 doi:10.1186/gb-2007-8-9-r188