Comparison of M0 and M24 enriched transcripts to HLH-1 induced muscle genes. Embryos in which most blastomeres have been converted to muscle-like cells by the induced expression of an hlh-1 transgene were profiled over time for gene expression . Data were obtained from the Affymetrix platform also used for the M0 and M24 profiles, allowing a direct comparison of the datasets. The Venn diagram shows the overlap between the M0 + M24 and the HLH-1 induced transcripts with at least a 1.7-fold increase in expression compared with the respective reference samples. Panels to the left show the time course of gene expression (GeneSpring software; Agilent) for three independent samples at each time point for the HLH-1 induced dataset. Line coloring in these graphs reflects the 6-hour value compared with the 0 hour value for each gene, as indicated by the color key. The 592 transcripts common to both experimental approaches are strong candidates for muscle specific genes; most of these show induction (up to 100-fold) in the HLH-1 induced dataset.
Fox et al. Genome Biology 2007 8:R188 doi:10.1186/gb-2007-8-9-r188