Induction of interferon response in two types of breast cancer cell lines. (a) MDA-MB231 cells were incubated in conditioned media from CCL-171 monoculture, MDA-MB231 monoculture, T47D monoculture, CCL-171/MDA-MB231 co-culture and CCL-171/T47D co-culture. OAS2 gene expression was determined by quantitative RT-PCR. The gene expression level of GAPDH was used for normalization between the samples. A strong induction of OAS2 by the supernatant from the CCL-171/MDA-MB231 co-culture can be seen in MDA-MB231. (b) Incubation of T47D cells with conditioned media from CCL-171 monoculture, MDA-MB231 monoculture, T47D monoculture, CCL-171/MDA-MB231 co-culture and CCL-171/T47D co-culture showed that only the CCL-171/MDA-MB231 co-culture supernatant induced OAS2 gene expression, although to a much lesser extent than in MDA-MB231 cells. (c) Gene expression levels of IFNβ were determined by quantitative RT-PCR. CCL-171 cells show much higher IFNβ expression levels when isolated by FACS after co-culture with MDA-MB231 than with T47D cells. Expression levels in tumor cells are shown as controls. The error bars show the standard deviation from the normalized mean.
Buess et al. Genome Biology 2007 8:R191 doi:10.1186/gb-2007-8-9-r191