In vivo observations of reporter constructs in control or dRfx-deficient Drosophila. (a) Schematic of two typical chordotonal organs of the Drosophila leg or antenna. The different segments of the dendrite and of the ciliated ending are shown. Sensory neurons have a single cilium (arrow) extending from their dendrite (arrowhead). (b) Live confocal image of GFP driven expression of osm-1 transgene in a control femur. (c) GFP expression is totally shut down in a dRfx mutant background. (d-i) Confocal imaging of chordotonal neurons labeled with anti-ELAV (red) and anti-GFP (green). oseg1-GFP expression in (d) control flies and (e) a dRfx mutant background. Note that oseg1-GFP expression is not affected in the mutant background. CG3259-GFP expression in (f) control flies and (g) dRfx mutant flies. Reporter construct expression is totally shut down in the mutant background. Johnston's organs from antennae of adult flies carrying CG9227-GFP transgenes in (h) control and (i) dRfx mutant pupae. Note the absence of expression in the mutant background. Scale bar = 10 μm.
Laurençon et al. Genome Biology 2007 8:R195 doi:10.1186/gb-2007-8-9-r195