Method

A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila

Ramanuj DasGupta¹ , Kent Nybakken² , Matthew Booker³ , Bernard Mathey-Prevot³ , Foster Gonsalves¹ , Binita Changkakoty¹ and Norbert Perrimon^3,4

¹  New York University School of Medicine/Cancer Institute, Department of Pharmacology, First Avenue, New York, NY 10016, USA

²  Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA, 02472, USA

³  Department of Genetics, Harvard Medical School, Avenue Louis Pasteur, Boston, MA 02115, USA

⁴  Howard Hughes Medical Institute, Harvard Medical School, Avenue Louis Pasteur, Boston, MA 02115, USA

author email corresponding author email

Genome Biology 2007, 8:R203doi:10.1186/gb-2007-8-9-r203

Published:	28 September 2007

Subject areas: Molecular biology, Genome studies, Development

Abstract

Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes.