Figure 5.

Hh assay timing. Hh signaling assays were conducted on identical assays plates with luciferase assays being scored after one or four days. (a) Comparison of RL counts when luciferase activity was assayed after one or four days in the presence of the indicated co-transfected dsRNA. (b) Comparison of firefly luciferase counts when luciferase activity was assayed after one or four days in the presence of the indicated co-transfected dsRNA. (c) Normalized luciferase values derived from the data in (a) and (b) of one-versus four-day assays. All luciferase reporter assays were performed in triplicate and error bars represent the standard error between the three data points.