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This article is part of the supplement: Transposons in vertebrate functional genomics

Review

Meganuclease and transposon mediated transgenesis in medaka

Clemens Grabher1* and Joachim Wittbrodt2

Author Affiliations

1 Pediatric Oncology, Dana-Farber Cancer Institute, Binney St, Boston, Massachusetts 02115, USA

2 Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr., Heidelberg, 69117, Germany

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Genome Biology 2007, 8(Suppl 1):S10  doi:10.1186/gb-2007-8-s1-s10

Published: 31 October 2007

Abstract

From among a plethora of various gene delivery methods, the researcher must choose the right one according to availability for a given species and the precise application the transgenic animal is intended for. Here we review the progress in meganuclease and Sleeping Beauty transposon mediated transgenesis over recent years with a focus on medaka and zebrafish. We present a side-by-side comparison of these two approaches based on their biologic properties and provide interesting perspectives for future experiments and applications, which are different for the two techniques because of their distinct modes of action.