Table 1

Comparison of gene knockout strategies in rodents

Criteria

Retroviral

ENU

Retrotransposon


Disrupt genes completely

Yes

No

Yes

Random mutation

No

Yes

Yes

High frequency mutation

Yes

Yes

Yes

Quickly map mutations

Yes

No

Yes

Efficiently create knockout animals

No

Yes

Yes


ENU, N-ethyl-N-nitrosourea.

Ostertag et al. Genome Biology 2007 8(Suppl 1):S16   doi:10.1186/gb-2007-8-s1-s16