This article is part of the supplement: Transposons in vertebrate functional genomics
Transposon mediated transgenesis in a marine invertebrate chordate: Ciona intestinalis
1 Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, 415-0025, Japan
2 Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
3 CREST, Japan Science and Technology Agency, Kawaguchi, Saitama, 330-0012, Japan
4 Sars International Centre for Marine Molecular Biology, University of Bergen, Thormøhlens Gate, 5008 Bergen, Norway
Genome Biology 2007, 8(Suppl 1):S3 doi:10.1186/gb-2007-8-s1-s3Published: 31 October 2007
Achievement of transposon mediated germline transgenesis in a basal chordate, Ciona intestinalis, is discussed. A Tc1/mariner superfamily transposon, Minos, has excision and transposition activities in Ciona. Minos enables the creation of stable transgenic lines, enhancer detection, and insertional mutagenesis.