This article is part of the supplement: Transposons in vertebrate functional genomics
Transposons as tools for enhancer trap screens in vertebrates
Institute of Molecular and Cell Biology, Biopolis Dr., Proteos, 138673, Singapore; Department of Biological Sciences, Science Drive 4, National University of Singapore, 117543 Singapore
Genome Biology 2007, 8(Suppl 1):S8 doi:10.1186/gb-2007-8-s1-s8Published: 31 October 2007
DNA transposons are efficient tools in transgenesis and have therefore become popular in the analysis of the regulatory genome in vertebrates via enhancer trap screens. Here, I discuss recent progress in this field of research, with a focus on the application of one of these transposons, namely the medaka fish derived Tol2, to enhancer trapping in zebrafish, and how this approach compares with others that have a similar objective.