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This article is part of the supplement: Transposons in vertebrate functional genomics

Review

Insertional mutagenesis strategies in zebrafish

Sridhar Sivasubbu1, Darius Balciunas2, Adam Amsterdam3 and Stephen C Ekker24*

Author Affiliations

1 Institute of Genomics and Integrative Biology, Council for Scientific and Industrial Research, Mall Road, Delhi 110007, India

2 University of Minnesota, Department of Genetics, Cell Biology and Development, Arnold and Mabel Beckman Center for Transposon Research, Church St SE, Minneapolis, Minnesota 55455, USA

3 Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Massachusetts Avenue, Cambridge, Massachusetts 02139, USA

4 Mayo Clinic College of Medicine, Department of Biochemistry and Molecular Biology, Rochester, Minnesota 55905, USA

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Genome Biology 2007, 8(Suppl 1):S9  doi:10.1186/gb-2007-8-s1-s9

Published: 31 October 2007

Abstract

We review here some recent developments in the field of insertional mutagenesis in zebrafish. We highlight the advantages and limitations of the rich body of retroviral methodologies, and we focus on the mechanisms and concepts of new transposon-based mutagenesis approaches under development, including prospects for conditional 'gene trapping' and 'gene breaking' approaches.