Transposon mediated transgenesis in a marine invertebrate chordate: Ciona intestinalis

Yasunori Sasakura¹ , Yuichi Oogai², Terumi Matsuoka², Nori Satoh²^,3 and Satoko Awazu⁴

¹Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, 415-0025, Japan

²Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan

³CREST, Japan Science and Technology Agency, Kawaguchi, Saitama, 330-0012, Japan

⁴Sars International Centre for Marine Molecular Biology, University of Bergen, Thormøhlens Gate, 5008 Bergen, Norway

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Genome Biology 2007, 8(Suppl 1):S3doi:10.1186/gb-2007-8-s1-s3


Published:	31 October 2007

Abstract

Achievement of transposon mediated germline transgenesis in a basal chordate, Ciona intestinalis, is discussed. A Tc1/mariner superfamily transposon, Minos, has excision and transposition activities in Ciona. Minos enables the creation of stable transgenic lines, enhancer detection, and insertional mutagenesis.