Figure 5.

Transient expression assay in chicken. A plasmid DNA containing the transposase cDNA under the control of a ubiquitous promoter (CAGGS) and a transposon-donor plasmid DNA containing a Tol2 construct with the CAGGS promoter and the gene encoding green fluorescent protein (GFP) are introduced into the somite of a chicken embryo by electroporation. The Tol2 construct is excised from the plasmid and integrated in the genome. GFP expression in the somites persists until late developmental stages [28].