Insertional mutagenesis strategies in zebrafish

Sridhar Sivasubbu¹, Darius Balciunas², Adam Amsterdam³ and Stephen C Ekker²^,4

¹Institute of Genomics and Integrative Biology, Council for Scientific and Industrial Research, Mall Road, Delhi 110007, India

²University of Minnesota, Department of Genetics, Cell Biology and Development, Arnold and Mabel Beckman Center for Transposon Research, Church St SE, Minneapolis, Minnesota 55455, USA

³Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Massachusetts Avenue, Cambridge, Massachusetts 02139, USA

⁴Mayo Clinic College of Medicine, Department of Biochemistry and Molecular Biology, Rochester, Minnesota 55905, USA

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Genome Biology 2007, 8(Suppl 1):S9doi:10.1186/gb-2007-8-s1-s9


Published:	31 October 2007

Abstract

We review here some recent developments in the field of insertional mutagenesis in zebrafish. We highlight the advantages and limitations of the rich body of retroviral methodologies, and we focus on the mechanisms and concepts of new transposon-based mutagenesis approaches under development, including prospects for conditional 'gene trapping' and 'gene breaking' approaches.