Additional data file 6.
(A) tag-130 gene exon/intron structure. The regions deleted in tag-130gk245 and tag-130OK1073 are indicated with orange bars. (B) An enlargement of the OK1073 deleted region and schematic diagrams of two mRNA forms detected in tag-130OK1073 mutants. The deleted region is indicated in orange. In isoform OK1073-A, which is the more abundant of the two forms, the remaining portion of intron 13 is not spliced out and is juxtaposed with the remaining portion of exon 17. Intron 13 contains a stop codon in this reading frame, which should cause truncation of the protein. In the other isoform (OK1073-B), the remaining portion of intron 13 is spliced out along with the remaining portion of exon 17 (and intron 17), juxtaposing exon 13 with exon 18. This changes the reading frame in exon 18, and should also result in premature truncation of the protein.
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Tomoyasu et al. Genome Biology 2008 9:R10 doi:10.1186/gb-2008-9-1-r10