Figure 6.

Example of a novel transcript detected by RACE sequencing. (a) Novel transcript 5NGSP2F8 (with consensus splice site) has a potential open reading frame of 142 amino acids; also, there is spliced expressed sequence tag (EST) evidence for it. (b) Real-time PCR relative quantification of the novel transcript to HPRT1 in placenta polyA+ RNA. RACE, rapid amplification of cDNA ends.