Table 1

Summary of RACE sequencing using polyA+ and total RNA from human cell lines and tissue

Experiment
Number of exon primers
Number of novel TAR primers
Number of nonTx primers
Number of sequence reads
Number of detected transcripts on the genome

1: NB4 total RNA
34
39
0
291
154
2: Hela polyA RNA
0
59
0
273
112
3: placenta total RNA
32
20
44
195
85
4: placenta polyA RNA
0
96
96
591
147

nonTx, region not previously shown to be transcribed; RACE, rapid amplification of cDNA ends; TAR, transcriptionally active region.

Wu et al. Genome Biology 2008 9:R3   doi:10.1186/gb-2008-9-1-r3