Table 1

Summary of RACE sequencing using polyA+ and total RNA from human cell lines and tissue

Experiment

Number of exon primers

Number of novel TAR primers

Number of nonTx primers

Number of sequence reads

Number of detected transcripts on the genome

1: NB4 total RNA

34

39

0

291

154

2: Hela polyA RNA

0

59

0

273

112

3: placenta total RNA

32

20

44

195

85

4: placenta polyA RNA

0

96

96

591

147

nonTx, region not previously shown to be transcribed; RACE, rapid amplification of cDNA ends; TAR, transcriptionally active region.

Wu et al. Genome Biology 2008 9:R3   doi:10.1186/gb-2008-9-1-r3