Research
Rapid identification of PAX2/5/8 direct downstream targets in the otic vesicle by combinatorial use of bioinformatics tools
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* Corresponding author: Joachim Wittbrodt Jochen.Wittbrodt@EMBL-heidelberg.de
1 Developmental Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117-Heidelberg, Germany
2 Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria
3 European Bioinformatics Institute, Wellcome Trust Genome Campus Hinxton, Cambridge, CB10 1SD, UK
4 University of Applied Sciences FH Campus Wien, Viehmarktgasse 2A, 1030 Vienna, Austria
5 Current Address: Max-Planck Institute of Immunbiology, Stübeweg 51, 79108-Freiburg, Germany
Genome Biology 2008, 9:R145 doi:10.1186/gb-2008-9-10-r145
Published: 1 October 2008Additional files
Additional data file 1:
Candidate genes from the EDF pipeline (matrices A and B).
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Additional data file 2:
The genes highlighted in color are those that were subjected to experimental validation in medaka. Green: genes specifically expressed in the otic vesicle and affected by Pax2/8 overexpression (see also Figure 3). Blue: genes expressed in the otic vesicle but not affected by Pax2/8 overexpression (see also Additional data file 3). Pink: genes not expressed in the otic vesicle.
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Additional data file 3:
Dorso-lateral views of medaka otic vesicle, anterior is towards the left. Developmental stages (Stg) are indicated for each embryo. All four candidates exhibit a weak expression in the otic epithelium.
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Additional data file 4:
l2hgdh: L-2-hydroxyglutarate dehydrogenase, mitochondrial precursor, c22orf28: unknown protein, tacstd2: tumor-associated calcium signal transducer 2 precursor.
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