Figure 7.

NAD⁺ biosynthetic genes and Sirtuin function. (a) Six-fold serial dilutions of the indicated strains were spotted onto YEPD plates and grown for 3 days at the indicated temperatures before being photographed. WT, wild type. (b) Six-fold serial dilutions of the indicated strains were spotted onto YEPD plates and grown for 3 days at the indicated temperatures before being photographed. (c) Six-fold serial dilutions of the indicated strains were spotted onto YEPD plates, YEPD plates containing 7.5 mM nicotinamide and YEPD plates containing 20 mM isonicotinamide, and grown for 3 days at the indicated temperatures before being photographed. (d) NAD⁺ levels in indicated strains; values represent the mean of two measurements. (e) NAD⁺ levels in indicated strains; values represent the mean of two measurements.