Table 1 |
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|
An example of using peptide ratios used to derive protein relative expression results (odorant binding protein 14 [GenBank:94158822]) |
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|
Peptide |
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|
|
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|
Sample |
Larval ages compared |
Replicate (1, 2, or 3) |
1 |
2 |
3 |
4 |
5 |
Ln (peptide average) |
Ln (protein average) |
|
|
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|
H |
1, 3 |
1 |
-1.26 |
-1.41 |
-1.66 |
-1.82 |
-2.25 |
-1.68 |
-3.01 |
|
H |
2 |
-3.29 |
-3.39 |
-3.55 |
-3.68 |
-3.74 |
-3.53 |
||
|
H |
3 |
-3.76 |
-3.82 |
-3.82 |
-3.82 |
-3.82 |
-3.81 |
||
|
H |
2, 3 |
1 |
-0.68 |
-0.93 |
-1.28 |
-1.32 |
-1.77 |
-1.20 |
-1.35 |
|
H |
2 |
-0.85 |
-0.97 |
-1.06 |
-1.13 |
-1.62 |
-1.12 |
||
|
H |
3 |
-1.57 |
-1.59 |
-1.69 |
-2.11 |
NA |
-1.74 |
||
|
H |
4, 3 |
1 |
3.91 |
3.91 |
3.91 |
3.91 |
NA |
3.91 |
2.22 |
|
H |
2 |
-0.10 |
0.53 |
0.91 |
1.94 |
3.10 |
1.28 |
||
|
H |
3 |
0.67 |
1.56 |
1.60 |
1.69 |
1.84 |
1.47 |
||
|
H |
5, 3 |
1 |
3.91 |
3.91 |
3.91 |
3.91 |
3.91 |
3.91 |
3.09 |
|
H |
2 |
2.16 |
2.32 |
2.50 |
3.04 |
3.04 |
2.61 |
||
|
H |
3 |
2.05 |
2.55 |
2.57 |
2.78 |
3.79 |
2.75 |
||
|
T |
1, 3 |
1 |
-1.29 |
-1.37 |
-1.57 |
-1.80 |
-1.80 |
-1.57 |
-0.53 |
|
T |
2 |
-1.68 |
-2.16 |
-2.16 |
NA |
NA |
-2.00 |
||
|
T |
3 |
1.47 |
1.83 |
2.59 |
NA |
NA |
1.96 |
||
|
T |
2, 3 |
1 |
-0.55 |
-0.58 |
-0.78 |
-0.91 |
-0.93 |
-0.75 |
-0.72 |
|
T |
2 |
-0.34 |
-0.42 |
-0.55 |
-1.04 |
-1.42 |
-0.75 |
||
|
T |
3 |
-0.46 |
-0.66 |
-0.86 |
NA |
NA |
-0.66 |
||
|
T |
4, 3 |
1 |
-0.39 |
-0.40 |
-0.69 |
-0.70 |
-0.76 |
-0.59 |
-0.37 |
|
T |
2 |
-0.06 |
0.24 |
0.38 |
0.70 |
0.81 |
0.41 |
||
|
T |
3 |
-0.65 |
-0.95 |
-1.02 |
-1.07 |
NA |
-0.92 |
||
|
T |
5, 3 |
1 |
-0.02 |
-0.05 |
-0.27 |
-0.31 |
-0.40 |
-0.21 |
0.76 |
|
T |
2 |
0.37 |
0.67 |
0.90 |
1.47 |
NA |
0.85 |
||
|
T |
3 |
0.80 |
1.53 |
1.67 |
2.04 |
2.08 |
1.62 |
||
|
|
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|
Both the honey bee larval hemolymph (H) and tissue (T) samples were collected daily for 5 days post-hatching, and peptides from days 1, 2, 4, and 5 were isotopically labeled and mixed at 1:1 (by protein amount) with day 3 peptides, which were labeled differentially from the other days. Relative peptide intensities were recorded (limited at 50-fold or 3.91 in natural log (Ln)) and proteins with a minimum of 2 quantified peptides were natural log-transformed and averaged; for proteins with greater than 5 peptides, the top 5 most intense ones were selected for averaging. In samples where there were less than 5 peptides, their absence is indicated by NA (not available). |
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|
Chan and Foster Genome Biology 2008 9:R156 doi:10.1186/gb-2008-9-10-r156 |
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