Research
Egr1 regulates the coordinated expression of numerous EGF receptor target genes as identified by ChIP-on-chip
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* Corresponding author: Dan Mercola dmercola@uci.edu
1 Department of Pathology and Laboratory Medicine, University of California, Irvine, CA 92697, USA
2 Sidney Kimmel Cancer Center, San Diego, CA 92121, USA
3 Department of Periodontology, School of Dentistry, Ege University, Bornova 35100, Izmir, Turkey
4 Burnham Institute for Medical Research, La Jolla, CA 92037, USA
5 National Institutes of Health; Cell and Cancer Biology Branch, National Cancer Institute, Bethesda, MD 20892, USA
Genome Biology 2008, 9:R166 doi:10.1186/gb-2008-9-11-r166
Published: 25 November 2008Additional files
Additional data file 1:
Construction of the promoter arrays, hybridization and analysis of the promoter arrays.
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Additional data file 2:
Identified targets of Egr1.
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Additional data file 3:
Table S2: log fold changes and p-values of the Egr1 target genes that were significantly differentially expressed between UV treated and mock cells using Affymetrix HGU133plus 2 arrays. Table S3: the gene expression analysis of Egr1 target genes using qRT-PCR of RNA extracted at various time points after UV-C treatment of DU145 cells.
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Additional data file 4:
Figure S1: diagram showing previously reported interactions between EGFR and 23 other Egr1 target genes using Pathway Studio (Ariadne Inc.). Figure S2: plot showing the distribution of fold change values from Affymetrix gene expression data and M values from ChIP-on-chip data for Egr1 target genes. Figure S3: diagram showing previously reported interactions between Egr1's target genes using Pathway Studio (Ariadne Inc.). Figure S4: gene expression of p53, p73, TGFb1 and PTEN was studied by qRT-PCR analysis of RNA isolated from M12 cells at various time points after UV-C treatment.
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