Figure 5.

Assay of putative CNCS promoters. (a) Bar graph showing the normalized luciferase activity of putative promoter CNCSs in an episomal vector without minimal promoter. Bidirectionality was tested by cloning the sequences in the native or reverse orientation. Broken bars show values that are off scale. All CNCSs overlapping DHSs are included. (b) Pie chart showing the proportion of random CNCSs with enhancer, silencing, promoter or no activity.