Table 2 |
||||||||||||||||||
|
Protein data for the 54 protein isoforms identified in this study |
||||||||||||||||||
|
Protein name |
PlasmoDB ID and NCBI GenBank accession number |
Calculated |
Spot number |
Mascot MS/MS ion search |
Average volume ratio |
Relative standard deviation |
Maximum fold change |
One-way ANOVA P value |
||||||||||
|
|
|
|
|
|||||||||||||||
|
Mass (kDa) |
pI |
Score |
Peptides matched |
Sequence coverage |
TP1 |
TP2 |
TP3 |
TP4 |
TP1 |
TP2 |
TP3 |
TP4 |
Median |
|||||
|
|
||||||||||||||||||
|
2-Cys peroxiredoxin |
[PDB:PF14_0368] [Genbank:AAN36981] |
22.0 |
6.7 |
- |
559 |
4 |
48% |
1.03 |
1.02 |
0.96 |
0.78 |
5.3% |
15.4% |
9.9% |
6.4% |
8.1% |
1.33× |
0.016 |
|
|
||||||||||||||||||
|
Actin-I |
[PDB:PFL2215w] [Genbank:AAN36527] |
42.1 |
5.2 |
1 |
533 |
9 |
40% |
0.58 |
0.97 |
1.30 |
1.24 |
5.9% |
5.9% |
8.5% |
3.2% |
5.9% |
2.24× |
<0.001 |
|
2 |
609 |
8 |
37% |
0.60 |
0.93 |
1.44 |
1.25 |
4.8% |
5.6% |
5.6% |
3.4% |
5.2% |
2.39× |
<0.001 |
||||
|
3 |
391 |
8 |
36% |
0.66 |
1.01 |
1.60 |
1.35 |
7.0% |
4.4% |
7.0% |
2.2% |
5.7% |
2.43× |
<0.001 |
||||
|
4 |
415 |
5 |
21% |
0.63 |
0.94 |
1.21 |
0.97 |
7.7% |
3.4% |
8.6% |
6.8% |
7.3% |
1.91× |
<0.001 |
||||
|
5 |
192 |
6 |
29% |
1.57 |
0.73 |
0.78 |
1.40 |
8.7% |
7.0% |
12.6% |
11.3% |
10.0% |
2.15× |
<0.001 |
||||
|
|
||||||||||||||||||
|
Adenosine deaminase |
[PDB:PF10_0289] [Genbank:AAN35486] |
42.9 |
5.4 |
- |
484 |
7 |
23% |
1.24 |
1.18 |
1.15 |
0.80 |
14.5% |
5.0% |
2.6% |
6.6% |
5.8% |
1.55× |
<0.001 |
|
|
||||||||||||||||||
|
eIF4A/RNA helicase-1/helicase 45 |
[PDB:PF14_0655] [Genbank:AAN37268] |
45.3 |
5.5 |
1 |
318 |
8 |
24% |
0.41 |
1.32 |
1.19 |
1.01 |
4.4% |
3.7% |
7.8% |
5.8% |
5.1% |
3.26× |
<0.001 |
|
2 |
159 |
2 |
10% |
0.61 |
1.24 |
1.23 |
1.05 |
5.6% |
5.0% |
6.6% |
2.7% |
5.3% |
2.03× |
<0.001 |
||||
|
3 |
143 |
6 |
22% |
0.73 |
1.20 |
1.30 |
1.16 |
8.8% |
6.1% |
20.6% |
9.5% |
9.2% |
1.79× |
<0.001 |
||||
|
4 |
133 |
4 |
19% |
1.58 |
0.57 |
0.88 |
1.11 |
5.7% |
5.2% |
8.2% |
3.6% |
5.4% |
2.78× |
<0.001 |
||||
|
5 |
242 |
4 |
13% |
1.64 |
0.62 |
0.94 |
1.22 |
9.6% |
6.5% |
11.8% |
10.4% |
10.0% |
2.65× |
<0.001 |
||||
|
|
||||||||||||||||||
|
eIF4A-like helicase |
[PDB:PFB0445c] [Genbank:AAC71878] |
52.6 |
5.7 |
1 |
233 |
4 |
15% |
0.99 |
1.05 |
1.24 |
1.22 |
7.9% |
8.8% |
15.7% |
7.4% |
8.3% |
1.25× |
0.024 |
|
2 |
368 |
7 |
26% |
1.02 |
1.06 |
1.18 |
1.13 |
7.8% |
4.2% |
7.6% |
7.3% |
7.4% |
1.16× |
0.045 |
||||
|
|
||||||||||||||||||
|
eIF5A |
[PDB:PFL0210c] [Genbank:AAN36131] |
17.8 |
5.4 |
1 |
117 |
2 |
27% |
0.12 |
0.32 |
1.26 |
1.79 |
18.2% |
18.1% |
10.9% |
23.4% |
18.2% |
15.09× |
<0.001 |
|
2 |
129 |
2 |
16% |
1.05 |
0.98 |
0.79 |
0.55 |
4.9% |
12.3% |
30.1% |
28.8% |
20.5% |
1.9× |
0.005 |
||||
|
3 |
151 |
2 |
16% |
1.27 |
1.44 |
1.09 |
0.65 |
19.4% |
24.0% |
16.9% |
21.6% |
20.5% |
2.23× |
<0.001 |
||||
|
|
||||||||||||||||||
|
Enolase |
[PDB:PF10_0155] [Genbank:AAN35353] |
48.6 |
6.2 |
1 |
344 |
10 |
30% |
1.10 |
1.11 |
1.07 |
0.88 |
4.2% |
4.4% |
4.6% |
7.2% |
4.5% |
1.26× |
<0.001 |
|
2 |
513 |
10 |
33% |
1.15 |
1.18 |
0.99 |
0.83 |
14.1% |
9.5% |
4.8% |
10.0% |
9.8% |
1.42× |
<0.001 |
||||
|
3 |
627 |
10 |
33% |
1.18 |
1.17 |
1.09 |
0.87 |
5.4% |
5.0% |
7.9% |
8.5% |
6.7% |
1.35× |
<0.001 |
||||
|
4 |
897 |
10 |
39% |
1.13 |
1.06 |
1.11 |
0.92 |
11.7% |
2.2% |
8.3% |
5.2% |
6.7% |
1.22× |
0.016 |
||||
|
5 |
138 |
4 |
15% |
1.03 |
0.91 |
1.14 |
0.93 |
19.9% |
7.8% |
9.9% |
11.9% |
10.9% |
1.25× |
0.125 |
||||
|
3a |
213 |
5 |
14% |
1.83 |
1.09 |
0.68 |
0.52 |
12.7% |
12.9% |
24.3% |
3.3% |
12.8% |
3.52× |
<0.001 |
||||
|
4a |
122 |
3 |
11% |
1.43 |
1.06 |
0.85 |
0.73 |
0.9% |
3.1% |
16.0% |
4.8% |
4.0% |
1.94× |
<0.001 |
||||
|
|
||||||||||||||||||
|
EXP-2 |
[PDB:PF14_0678] [Genbank:AAN37291] |
33.6 |
5.1 |
- |
133 |
3 |
12% |
0.27 |
0.70 |
0.88 |
0.97 |
8.1% |
4.3% |
10.4% |
9.3% |
8.7% |
3.62× |
<0.001 |
|
|
||||||||||||||||||
|
HSP40 |
[PDB:PFE0055c] [PDB:MAL5P1.12] [Genbank:CAD51377] |
47.8 |
8.1 |
- |
118 |
4 |
19% |
0.70 |
0.85 |
0.94 |
2.06 |
13.2% |
37.4% |
14.2% |
18.9% |
16.5% |
2.94× |
<0.001 |
|
|
||||||||||||||||||
|
HSP60 |
[PDB:PF10_0153] [Genbank:AAN35351] |
62.9 |
6.7 |
1 |
171 |
5 |
11% |
1.29 |
0.96 |
1.10 |
1.37 |
8.6% |
4.1% |
7.0% |
3.8% |
5.5% |
1.43× |
<0.001 |
|
2 |
235 |
5 |
11% |
1.31 |
0.89 |
1.08 |
1.35 |
5.9% |
3.8% |
5.5% |
6.5% |
5.7% |
1.51× |
<0.001 |
||||
|
|
||||||||||||||||||
|
HSP70-1a |
[PDB:PF08_0054] [Genbank:CAD51185] |
74.4 |
5.5 |
1 |
607 |
10 |
20% |
1.31 |
1.13 |
1.01 |
0.93 |
14.7% |
10.1% |
12.9% |
7.9% |
11.5% |
1.41× |
0.008 |
|
2 |
644 |
10 |
22% |
1.46 |
1.23 |
1.06 |
0.96 |
5.7% |
4.5% |
10.8% |
7.1% |
6.4% |
1.52× |
<0.001 |
||||
|
|
||||||||||||||||||
|
HSP70-2a |
[PDB:PFI0875w] [Genbank:CAD51861] |
72.5 |
5.2 |
1 |
486 |
8 |
17% |
0.95 |
1.15 |
0.98 |
1.08 |
8.7% |
5.0% |
7.8% |
8.1% |
8.0% |
1.2× |
0.014 |
|
2 |
724 |
10 |
23% |
1.06 |
1.07 |
1.02 |
1.11 |
8.6% |
4.8% |
7.2% |
4.4% |
6.0% |
1.08× |
0.369 |
||||
|
3 |
694 |
10 |
21% |
1.26 |
0.87 |
1.03 |
1.09 |
4.9% |
2.5% |
6.1% |
4.1% |
4.5% |
1.46× |
<0.001 |
||||
|
|
||||||||||||||||||
|
HSP70-3a |
[PDB:PF11_0351] [Genbank:AAN35935] |
73.7 |
6.5 |
1 |
193 |
5 |
7% |
1.08 |
0.97 |
0.97 |
1.06 |
7.2% |
7.8% |
16.2% |
4.9% |
7.5% |
1.11× |
0.299 |
|
2 |
488 |
8 |
12% |
1.01 |
0.98 |
1.16 |
1.11 |
8.0% |
2.2% |
11.3% |
6.2% |
7.1% |
1.19× |
0.028 |
||||
|
|
||||||||||||||||||
|
CA1 |
- [Genbank:NP_001729] |
28.9 |
6.6 |
1 |
264 |
5 |
32% |
1.48 |
0.98 |
0.60 |
0.59 |
6.2% |
4.3% |
13.7% |
3.8% |
5.2% |
2.5× |
<0.001 |
|
2 |
412 |
7 |
40% |
1.16 |
0.94 |
0.72 |
0.77 |
6.0% |
7.1% |
17.4% |
6.0% |
6.6% |
1.61× |
<0.001 |
||||
|
|
||||||||||||||||||
|
Human SOD1 |
- [Genbank:NP_000445] |
16.2 |
5.7 |
- |
247 |
5 |
57% |
1.04 |
1.18 |
1.13 |
0.74 |
9.2% |
12.5% |
7.3% |
16.2% |
10.8% |
1.6× |
0.001 |
|
|
||||||||||||||||||
|
HETK |
[PDB:PFF1335c] [PDB:MAL6P1.153] [Genbank:CAG25088] |
20.6 |
7.0 |
- |
575 |
8 |
56% |
1.02 |
1.13 |
0.96 |
0.74 |
1.9% |
9.0% |
5.9% |
7.5% |
6.7% |
1.53× |
<0.001 |
|
|
||||||||||||||||||
|
Hypothetical protein PF10_0325 |
[PDB:PF10_0325] [Genbank:AAN35522] |
33.2 |
5.6 |
1 |
380 |
7 |
33% |
1.08 |
1.11 |
1.04 |
0.93 |
1.5% |
7.0% |
11.7% |
14.8% |
9.4% |
1.2× |
0.107 |
|
2 |
497 |
8 |
33% |
1.13 |
1.09 |
0.99 |
0.88 |
5.8% |
4.9% |
18.7% |
17.9% |
11.9% |
1.28× |
0.097 |
||||
|
|
||||||||||||||||||
|
Hypothetical protein PFF1295w |
[PDB:PFF1295w] [Genbank:CAG25080] |
43.9 |
7.2 |
- |
245 |
5 |
20% |
1.06 |
1.04 |
0.95 |
0.91 |
2.2% |
7.1% |
13.7% |
5.0% |
6.0% |
1.16× |
0.072 |
|
|
||||||||||||||||||
|
Hypothetical protein PFI1270w |
[PDB:PFI1270w] [Genbank:CAD51940] |
24.9 |
5.5 |
1 |
332 |
6 |
21% |
1.31 |
0.98 |
1.01 |
1.00 |
11.6% |
8.7% |
10.1% |
14.3% |
10.8% |
1.34× |
0.012 |
|
2 |
74 |
3 |
17% |
0.93 |
0.97 |
1.36 |
1.65 |
8.6% |
16.8% |
11.6% |
9.8% |
10.7% |
1.77× |
<0.001 |
||||
|
|
||||||||||||||||||
|
M1AP |
[PDB:MAL13P1.56] [Genbank:CAD52253] |
126.6 |
7.3 |
- |
171 |
7 |
10% |
1.47 |
0.91 |
0.87 |
0.89 |
12.2% |
8.7% |
13.4% |
10.9% |
11.5% |
1.69× |
<0.001 |
|
|
||||||||||||||||||
|
OAT |
[PDB:PFF0435w] [PDB:MAL6P1.91] |
46.0 |
6.5 |
- |
589 |
9 |
29% |
1.20 |
1.05 |
1.06 |
1.06 |
0.9% |
5.8% |
12.0% |
5.4% |
5.6% |
1.14× |
0.116 |
|
|
||||||||||||||||||
|
PEMT |
[PDB:MAL13P1.214] [Genbank:CAD52560] |
31.3 |
5.4 |
1 |
790 |
9 |
46% |
1.07 |
1.12 |
1.09 |
0.89 |
7.2% |
10.3% |
9.6% |
16.7% |
9.9% |
1.26× |
0.049 |
|
2 |
182 |
3 |
14% |
0.76 |
0.97 |
1.13 |
1.05 |
6.2% |
5.8% |
14.0% |
16.3% |
10.1% |
1.49× |
0.003 |
||||
|
|
||||||||||||||||||
|
Proteasome component C8 |
[PDB:PFC0745c] [Genbank:CAB11152] |
29.7 |
6.4 |
1 |
67 |
1 |
7% |
1.17 |
1.20 |
1.12 |
0.83 |
12.9% |
3.1% |
5.1% |
7.6% |
6.4% |
1.44× |
<0.001 |
|
2 |
275 |
4 |
28% |
0.98 |
0.96 |
0.96 |
0.82 |
7.4% |
9.2% |
10.8% |
11.2% |
10.0% |
1.2× |
0.072 |
||||
|
|
||||||||||||||||||
|
TCP1a |
[PDB:PF11_0331] [Genbank:AAN35915] |
60.6 |
6.7 |
- |
110 |
5 |
11% |
1.05 |
0.97 |
1.04 |
1.04 |
3.0% |
4.1% |
6.4% |
6.2% |
5.1% |
1.08× |
0.190 |
|
|
||||||||||||||||||
|
TPI |
[PDB:PF14_0378] [Genbank:AAN36991] |
28.1 |
6.0 |
1 |
579 |
8 |
43% |
1.18 |
1.13 |
1.04 |
0.86 |
6.1% |
4.5% |
11.8% |
9.4% |
7.7% |
1.37× |
<0.001 |
|
2 |
578 |
8 |
45% |
1.20 |
1.11 |
1.07 |
0.88 |
7.0% |
5.1% |
10.0% |
9.6% |
8.3% |
1.37× |
<0.001 |
||||
|
|
||||||||||||||||||
|
Uridine phosphorylase |
[PDB:PFE0660c] [Genbank:CAD51497] |
27.5 |
6.1 |
- |
430 |
7 |
35% |
1.23 |
1.16 |
1.09 |
0.73 |
5.2% |
3.6% |
7.0% |
6.5% |
5.8% |
1.68× |
<0.001 |
|
|
||||||||||||||||||
|
The average volume ratios represent log2-transformed values and have not been mean-centered around zero. aNomenclature in agreement with Shonhai and coworkers [35]. CA1, human carbonic anhydrase 1; eIF, eukaryotic initiation factor; EXP, exported protein; HETK, hydroxyethylthiazol kinase; HSP, heat shock protein; M1AP, M1-family aminopeptidase; OAT, ornithine aminotransferase; PEMT, phosphoethanolamine N-methyltransferase; SOD, superoxide dismutase; TCP1a, T-complex protein 1, α subunit; TPI, triose phosphate isomerase. |
||||||||||||||||||
|
Foth et al. Genome Biology 2008 9:R177 doi:10.1186/gb-2008-9-12-r177 |
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