Figure 9.

Whole mount in situ hybridization analysis in mouse embryos shows expression in the developing facial prominences. Embryos at a range of mid-gestational stages were hybridized with an antisense ribroprobe to the 3' untranslated region of Baz1b. (a-e) Views of whole embryos from 8.25-11.5 dpc reveal strong expression in the mesenchyme of the maxillary and mandibular prominences, branchial arch 2 and the nasal processes. Other sites of Baz1b expression include the presumptive hindbrain, headfolds and tailbud at 8.5 dpc, and the limb mesenchyme and somites at later stages. (f) Higher magnification dorsal view of presumptive hindbrain staining at 8.25 dpc. Close up images of facial prominence staining are shown at (g) 8.75 dpc, (h) 10.5 dpc, and (i) 11.5 dpc. Images shown in (h, i) are ventral views following dissection of the head from the body of the embryo. BA1, branchial arch 1; BA2, branchial arch 2; Fl, forelimb; Fnp, frontonasal process; Hb, hindbrain; Hf, headfold; Hl, hindlimb; Lnp, lateral nasal prominence; Md, mandibular prominence; Mnp, medial nasal prominence; Mx, maxillary prominence; R, rhombomere; S, somite; Tb, tailbud.