Spurious intensity-dependent correlations between pairs of genes and between genes and bias metrics. Probe sets were sorted by mean expression and divided into 50 bins of approximately 250 probe sets each. For each possible pair of bins, the median correlation coefficient between probe sets is indicated by color. Thus, the presence of colored regions indicates intensity-dependent correlation bias between pairs of probe sets. (a) In the uncorrected data, the correlation between a given pair of probe sets is biased by the mean expression value of each probe set. For example, two probe sets that are both highly expressed on average are more likely to be positively correlated than expected by chance. (b) In the bias-corrected data, the correlation bias is reduced.
Eklund and Szallasi Genome Biology 2008 9:R26 doi:10.1186/gb-2008-9-2-r26