Temporal regulation of expression of immediate early and second phase transcripts by endothelin-1 in cardiomyocytes

Timothy E Cullingford¹ , Thomais Markou¹ , Stephen J Fuller¹ , Alejandro Giraldo¹ , Sampsa Pikkarainen¹ , Georgia Zoumpoulidou¹ , Ali Alsafi¹ , Collins Ekere¹ , Timothy J Kemp¹ , Jayne L Dennis² , Laurence Game² , Peter H Sugden¹ and Angela Clerk¹

¹National Heart and Lung Institute Division, Faculty of Medicine, Imperial College London, Armstrong Road, London SW7 2AZ, UK

²Clinical Sciences Centre/Imperial College Microarray Centre, Faculty of Medicine, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK

author email corresponding author email

Genome Biology 2008, 9:R32doi:10.1186/gb-2008-9-2-r32


Published:	14 February 2008

Subject areas: Cell biology, Genome studies

Additional files

Additional data file 1:

Variably expressed genes classified according to function and clustered according to time.

Format: XLS Size: 295KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional data file 2:

SQPCR and QPCR validation data for the RNAs that were upregulated by ET-1 at 30 minutes, and the effects of (a) cycloheximide or (b) U0126.

Format: DOC Size: 103KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional data file 3:

Data previously reported for the changes in RNA expression induced by ET-1 at 2 or 4 h are from [24].

Format: XLS Size: 45KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional data file 4:

RNAs that were upregulated by ET-1 at 1 or 2 h, clustered according to the effects of cycloheximide.

Format: XLS Size: 141KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional data file 5:

RNAs that were variably expressed at 1 h, clustered according to their distribution in polysome or total RNA pools.

Format: XLS Size: 89KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional data file 6:

QPCR data for validation of cycloheximide and polysome clusters.

Format: DOC Size: 128KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional data file 7:

Primers used for SQPCR or QPCR.

Format: DOC Size: 126KB Download file

This file can be viewed with: Microsoft Word Viewer