Table 2

Leishmania candidate virulence factors enriched in Cm

GeneDB accession numbers

Protein identification^a

Mean Cm/CA ratio^b

Putative function^c

Signal transduction proteins

LmjF35.2420

Phosphoinositide-binding protein, putative

3.51

Kinase^d

LmjF33.1380

Mitogen-activated protein kinase 11, putative, MAP kinase, putative

2.42

Kinase

LmjF09.0770

Oligopeptidase b, serine peptidase, clan SC, family S9A-like protein

2.31

Cell-cell signaling

LmjF34.2820

Regulatory subunit of protein kinase a-like protein

2.30

Kinase

LmjF28.2740

Activated protein kinase c receptor (LACK)

2.28

Kinase receptor

LmjF25.0750

Protein phosphatase, putative

2.27

Phosphatase

LmjF35.1010

Casein kinase, putative

2.08

Kinase

LmjF10.0490

Mitogen-activated protein kinase 3, putative, MAP kinase 3, putative

1.73

Kinase, signal transduction

LmjF31.2790

ADP-ribosylation factor, putative

1.67

Small GTPase mediated signal transduction

Immunosupressive proteins

LmjF35.2210

Kinetoplastid membrane protein-11

2.33

Immunosuppressive [51]

LmjF33.1750

Macrophage migration inhibitory factor-like protein

2.21

Immunosuppressive [52]

LmjF25.0910

Cyclophilin a

1.73

Immunosuppressive [103]

Proteins Involved in Intracellular survival

LmjF14.1360

Myo-inositol-1-phosphate synthase

3.93

Inositol biosynthesis [50]

LmjF23.0200

Endoribonuclease L-PSP (pb5), putative

3.91

mRNA salvage, inhibition protein synthesis

LmjF11.0630

Aminopeptidase, putative, metallo-peptidase, clan MF, family M17

3.05

Proteolysis

LmjF28.1730

Proteasome regulatory non-ATP-ase subunit 2, putative

2.40

Proteolysis

LmjF34.1040

Uracil phosphoribosyltransferase, putative

2.34

Pyrimidine salvage

LmjF09.0770

Oligopeptidase b, serine peptidase, clan SC, family S9A-like protein

2.31

Invasion, proteolysis

LmjF32.1820

Iron superoxide dismutase, putative

2.29

Antioxidant

LmjF13.0090

Carboxypeptidase, putative, metallo-peptidase, clan MA(E), family 32

2.14

Proteolysis

LmjF28.2770

Heat-shock protein hsp70, putative

2.14

Protein stability

LmjF26.1570

Thimet oligopeptidase, putative, metallo-peptidase, clan MA(E), family M3

2.10

Proteolysis

LmjF05.0960

Dipeptidyl-peptidase III, putative, metallo-peptidase, clan M-, family M49

2.08

Proteolysis

LmjF14.1160

Enolase

2.05

Plasminogen binding [104], Invasion

LmjF19.0160

Aminopeptidase, putative, metallo-peptidase, clan MG, family M24

2.04

Proteolysis

LmjF21.1830

Proteasome alpha 5 subunit, putative

2.03

Proteolysis

LmjF05.0350

Trypanothione reductase

1.99

Antioxidant

LmjF21.0760

Proteasome regulatory non-ATP-ase subunit 5, putative,19S proteasome regulatory subunit

1.97

Proteolysis

LmjF23.0270

Pteridine reductase 1

1.92

Antioxidant

LmjF26.0810

Glutathione peroxidase-like protein, putative

1.89

Antioxidant

LmjF27.0190

Proteasome alpha 7 subunit, putative

1.89

Proteolysis

LmjF26.2280

Nitrilase, putative

1.86

Carbon-nitrogen hydrolase

LmjF26.1240

Heat shock protein 70-related protein

1.86

Protein stability

LmjF31.1890

Peptidase m20/m25/m40 family-like protein

1.85

Proteolysis

LmjF06.0140

Proteasome beta 6 subunit, putative,20S proteasome beta 6 subunit, putative

1.85

Proteolysis

LmjF29.0120

Proteasome regulatory non-ATPase subunit, putative

1.81

Proteolysis

LmjF34.0650

Proteasome regulatory non-ATP-ase subunit 11, putative,19S proteasome regulatory subunit, metallo-peptidase, Clan MP, Family M67

1.79

Proteolysis

LmjF35.0750

Proteasome activator protein pa26, putative

1.78

Proteolysis

LmjF35.2350

Aminopeptidase P, putative, metallo-peptidase, clan MG, family M24

1.77

Proteolysis

LmjF02.0370

Proteasome regulatory non-ATPase subunit 6, putative

1.77

Proteolysis

LmjF36.3210

14-3-3 protein-like protein

1.74

Anti-apoptotic

LmjF21.1700

Proteasome alpha 2 subunit, putative

1.66

Proteolysis

LmjF36.1600

Proteasome alpha 1 subunit, putative

1.64

Proteolysis

LmjF35.4850

Proteasome alpha 1 subunit, putative

1.60

Proteolysis

Proteins involved in vesicular transport processes

LmjF35.2420

Phosphoinositide-binding protein, putative (sorting nexin 4)

3.51

Transport of proteins and other substances

LmjF27.0760

Small GTP-binding protein Rab1, putative

2.27

Endosomes/Golgi trafficking

LmjF32.1730

Coatomer epsilon subunit, putative

2.09

Intracellular protein transport

LmjF36.1630

Clathrin heavy chain, putative

1.98

Endocytosis, trans-Golgi to lysosome trafficking

LmjF18.0700

Hypothetical protein, conserved

1.85

HEAT repeat, intracellular protein transport

LmjF31.2790

ADP-ribosylation factor, putative

1.67

Intracellular protein transport

^aThe Mascot algorithm {2008 609/id} was used to identify the protein names and the GeneDB accession numbers [29]. ^bMean of normalized, log normal (Ln) transformed conditioned medium (Cm)/cell associated (CA) peptide ratios for at least three of four experiments. ^cPutative functions and locations are derived from the GeneDB database {2007 238/id} unless otherwise noted. ^dProtein sequence contains an amino-terminal secretion signal peptide according to SignalP.

Silverman et al. Genome Biology 2008 9:R35   doi:10.1186/gb-2008-9-2-r35