Open Access Research

Systems biology meets stress ecology: linking molecular and organismal stress responses in Daphnia magna

Lars-Henrik Heckmann12*, Richard M Sibly1, Richard Connon13, Helen L Hooper1, Thomas H Hutchinson45, Steve J Maund6, Christopher J Hill1, Anthony Bouetard1 and Amanda Callaghan1

  • * Corresponding author: Lars-Henrik Heckmann lhh@dmu.dk

Author Affiliations

1 University of Reading, School of Biological Sciences, Environmental Biology, Philip Lyle Building, Reading, RG6 6BX, UK

2 University of Aarhus, National Environmental Research Institute, Department of Terrestrial Ecology, Vejlsøvej, DK-8600, Silkeborg, Denmark

3 University of California, School of Veterinary Medicine, Department of Anatomy, Physiology and Cell Biology, Davis, California 95616, USA

4 AstraZeneca Global SHE, Brixham Environmental Laboratory, Devon, TQ5 8BA, UK

5 Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK

6 Syngenta Crop Protection AG, 4002 Basel, Switzerland

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Genome Biology 2008, 9:R40  doi:10.1186/gb-2008-9-2-r40

Published: 21 February 2008

Additional files

Additional data file 1:

Presented is a table listing all the cDNAs (annotated) that responded to ibuprofen treatment on the D. magna microarray.

Format: PDF Size: 121KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional data file 2:

Presented is a table showing the relative expression and GO of the unique D. magna genes responding to acute ibuprofen exposure.

Format: PDF Size: 109KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional data file 3:

Presented is a table displaying QPCR confirmation of selected D. magna genes responding on the cDNA microarray.

Format: PDF Size: 81KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional data file 4:

Presented is an image of a D. magna embryo arrested at developmental stage 1 to 2 after maternal exposure to ibuprofen.

Format: PDF Size: 216KB Download file

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Open Data

Additional data file 5:

Presented is a document with supplementary methods on microarray hybridization and microarray construction.

Format: PDF Size: 55KB Download file

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Open Data

Additional data file 6:

Presented are MA plots of raw and normalized microarray data

Format: PDF Size: 2.9MB Download file

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Open Data

Additional data file 7:

Presented is a table showing water chemical parameters measured during the follow-up experiments.

Format: PDF Size: 76KB Download file

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Open Data

Additional data file 8:

Presented is a table listing technical data on QPCR (for instance, primers and amplification efficiency) from the follow-up experiment assessing temporal expression of key genes responding to ibuprofen.

Format: PDF Size: 54KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data