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Systems biology meets stress ecology: linking molecular and organismal stress responses in Daphnia magna

Lars-Henrik Heckmann1,2 email, Richard M Sibly1 email, Richard Connon1,3 email, Helen L Hooper1 email, Thomas H Hutchinson4,5 email, Steve J Maund6 email, Christopher J Hill1 email, Anthony Bouetard1 email and Amanda Callaghan1 email

1University of Reading, School of Biological Sciences, Environmental Biology, Philip Lyle Building, Reading, RG6 6BX, UK

2University of Aarhus, National Environmental Research Institute, Department of Terrestrial Ecology, Vejlsøvej, DK-8600, Silkeborg, Denmark

3University of California, School of Veterinary Medicine, Department of Anatomy, Physiology and Cell Biology, Davis, California 95616, USA

4AstraZeneca Global SHE, Brixham Environmental Laboratory, Devon, TQ5 8BA, UK

5Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK

6Syngenta Crop Protection AG, 4002 Basel, Switzerland

author email corresponding author email

Genome Biology 2008, 9:R40doi:10.1186/gb-2008-9-2-r40

Published: 21 February 2008

Subject areas: Ecology, Genome studies, Medicine, Physiology


Additional files

Additional data file 1:

Presented is a table listing all the cDNAs (annotated) that responded to ibuprofen treatment on the D. magna microarray.

Format: PDF Size: 121KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional data file 2:

Presented is a table showing the relative expression and GO of the unique D. magna genes responding to acute ibuprofen exposure.

Format: PDF Size: 109KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional data file 3:

Presented is a table displaying QPCR confirmation of selected D. magna genes responding on the cDNA microarray.

Format: PDF Size: 81KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional data file 4:

Presented is an image of a D. magna embryo arrested at developmental stage 1 to 2 after maternal exposure to ibuprofen.

Format: PDF Size: 216KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional data file 5:

Presented is a document with supplementary methods on microarray hybridization and microarray construction.

Format: PDF Size: 55KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional data file 6:

Presented are MA plots of raw and normalized microarray data

Format: PDF Size: 3MB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional data file 7:

Presented is a table showing water chemical parameters measured during the follow-up experiments.

Format: PDF Size: 76KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional data file 8:

Presented is a table listing technical data on QPCR (for instance, primers and amplification efficiency) from the follow-up experiment assessing temporal expression of key genes responding to ibuprofen.

Format: PDF Size: 54KB Download file

This file can be viewed with: Adobe Acrobat Reader


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