Table 1

Functional categorization of Daphnia magna genes responding to acute ibuprofen exposure

Process
Functional category
Induced
Suppressed

1 Metabolism
1.1 Carbohydrate metabolism



     Glycolysis/Gluconeogenesis (PATH:00010)
Eno


     Fructose and mannose metabolism (PATH:00051)
MANA


     Starch and sucrose metabolism (PATH:00500)
AmyA, GlyPa


1.2 Energy metabolism



     Oxidative phosphorylation (PATH:000190)
atpBb, CYTB, RISP
ANT, CO1c, CO2c, ND4d

1.3 Lipid metabolism
GM2AP


     Glycerolipid metabolism (PATH:00561)
Lip


     Eicosanoid metabolism (PATH:00590)
Ltb4dh
GPXe

1.4 Amino acid metabolism



     Cysteine metabolism (PATH:00272)
Sult1C


1.5 Metabolisms of co-factors and vitamins



     Nicotinate and nicotinamide metabolism (PATH:000760)

Nt5f

1.6 Polypeptide metabolism (proteolysis)
BPTI
Spint

     Peptidases
CATL1, CPA2, Ctrb2, SC1, Try
ASTL, SP
2 Genetic information processing
2.1 Transcription

Ubn, H4

2.2 Translation
DEADc, Sep15


     Ribosome (PATH:03010)
16S rRNA, 28S rRNA, RpL6, RpL14, RpL15, RpL28, RpL30, RpL38, RpS2, RpS3A, RpS4, RpS12, RpS20, RpS25, RpS30
RpL9, RpL22, RpL27, RpS10, RpS13, RpS17

2.3 Chaperones and folding catalysts
HSP20, UBQ
PDI
3 Environmental information processing
3.1 Membrane transport
Inx2


3.2 Signal transduction
Ptn, Reep5


     MAPK signaling pathway (PATH:04010)
HSP70g


     Calcium signaling pathway (PATH:04020)
VDAC2


     Wnt signaling pathway (PATH:04310)
RhoAh
CTBPi, Skp1j

3.3 Signaling molecules and interaction

CNTN1

3.4 Sensory system
A10

4 Cellular processes
4.1 Cell motility
MRLC2, Tm1


4.2 Cell communication (PATH:01430)
Relnk
Acth

4.3 Endocrine system



     PPAR signaling pathway (PATH:03320)
FABP3
ACSl

     Oogenesis (vitellogenesis and oocyte maturation) maturation)
DmagVTG1
JHE, LPD_N, VMO1

     Moulting
Chtm, ChtBD2, Gasp, LPCP29, Peritrophin-A
cap-2, ChtBD4, CP7, DD5, PCP16.7

4.4 Immune system

CLECT, CUB, GNBP

4.5 Inorganic ion transport and metabolism
AT1A, CRIP, Fer, Fer1HCH, dmHb2, VGCa
dmHb1, Sfat, Znf_AN1

Genes presented in bold are directly linked to a Kyoto Encyclopedia of Genes and Genomes pathway, whereas other genes are listed with their functional category or subcategory based on Gene Ontology (see Additional data file 2). aGlyP is also associated with the insulin signaling pathway (PATH:04910). batpB is also associated with the type III secretion system (PATH:03070), flagellar assembly (PATH:02040), and epithelial cell signaling in Helicobacter pylori infection (PATH:05120). cCO1 and CO2 are also associated with the vascular endothelial growth factor signaling pathway (PATH:04370). dND4 is also associated with ubiquinone biosynthesis (PATH:00130). eGPX is also associated with glutathione metabolism (PATH:00480). fNt5 is also associated with purine metabolism (PATH:00230) and pyrimidine metabolism (PATH:00240). gHSP70 is also associated with antigen processing and presentation (PATH:004612) related to the immune system. hAct and RhoA are involved in eight and nine different pathways, respectively. Here they have been associated with one that is already represented, but because of this multi-alignment they have not been considered any further. iCTBP is also associated with the notch signaling pathway (PATH:04330). jSkp1 is also associated with the cell cycle (PATH:04110), ubiquitin-mediated proteolysis (PATH:04120), and transforming growth factor-β signaling pathway (PATH:04350), which all interlink through the Wnt signaling pathway. kReln is also associated with focal adhesion (PATH:04510) and extracellular matrix receptor interaction (PATH:04512). lACS is also associated with the adipocytokine signaling pathway (PATH:04920) and fatty acid metabolism (PATH:00071). mAlthough related to aminosugars metabolism (PATH:00530), Cht has been categorized with moulting because of the key role of the encoded enzyme (chitinase) in this process among arthropods. Full gene names are available in Additional data file 1. PPAR, peroxisome proliferator-activated receptor.

Heckmann et al. Genome Biology 2008 9:R40   doi:10.1186/gb-2008-9-2-r40