Method

UTILLdb, a Pisum sativum in silico forward and reverse genetics tool

Marion Dalmais^{* 1} , Julien Schmidt^{* 1} , Christine Le Signor^{* 2} , Francoise Moussy² , Judith Burstin² , Vincent Savois² , Gregoire Aubert² , Veronique Brunaud¹ , Yannick de Oliveira¹ , Cecile Guichard¹ , Richard Thompson² and Abdelhafid Bendahmane¹

¹Unité de Recherche en Génomique Végétale, UMR INRA-CNRS, Rue Gaston Crémieux, 91057 Evry Cedex, France

²INRA, Unite Mixte de Recherche en Génétique et Ecophysiologie des Légumineuses (INRA-ENESAD), Domaine d'Epoisses, 21110 Bretenières, France

author email corresponding author email* Contributed equally

Genome Biology 2008, 9:R43doi:10.1186/gb-2008-9-2-r43

Published:	26 February 2008

Subject areas: Genetics, Methods, Plant biology

Abstract

The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb, that contains phenotypic as well as sequence information on mutant genes. UTILLdb can be searched online for TILLING alleles, through the BLAST tool, or for phenotypic information about mutants by keywords.