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UTILLdb, a Pisum sativum in silico forward and reverse genetics tool

Marion Dalmais1, Julien Schmidt1, Christine Le Signor2, Francoise Moussy2, Judith Burstin2, Vincent Savois2, Gregoire Aubert2, Veronique Brunaud1, Yannick de Oliveira1, Cecile Guichard1, Richard Thompson2 and Abdelhafid Bendahmane1*

Author Affiliations

1 Unité de Recherche en Génomique Végétale, UMR INRA-CNRS, Rue Gaston Crémieux, 91057 Evry Cedex, France

2 INRA, Unite Mixte de Recherche en Génétique et Ecophysiologie des Légumineuses (INRA-ENESAD), Domaine d'Epoisses, 21110 Bretenières, France

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Genome Biology 2008, 9:R43  doi:10.1186/gb-2008-9-2-r43

Published: 26 February 2008

Abstract

The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb, that contains phenotypic as well as sequence information on mutant genes. UTILLdb can be searched online for TILLING alleles, through the BLAST tool, or for phenotypic information about mutants by keywords.