Establishment of pea EMS mutant library. Caméor seeds were EMS mutagenized. Out of 8,600 M1 plants self-fertilized in an insect-proof glasshouse, 4,817 produced more than 5 M2 seeds each. Four M2 seeds, referred to as A-D, per M1 parent were grown to maturity and scored for phenotypes. DNA was extracted from the plants referred to as A, which were left to set M3 seeds. As a backup, M3 seeds were harvested from the sister B plants. The collected M3 seeds were sent to the Grain Legumes Biological Resource Center for distribution, maintenance of the lines and long-term storage of the mutant library.
Dalmais et al. Genome Biology 2008 9:R43 doi:10.1186/gb-2008-9-2-r43