Figure 5.

TILLING screen. Example of a PsMetI TILLING screen on eightfold pooled pea DNA. The image of the cleavage reaction is collected from both channels (dyes IRD700 and IRD800). The sizes of the cleavage products (circled) from the two dye-labeled DNA strands (red or green) add up to the size of the full-length PCR product (top of the gel). PCR artifacts are distinguishable from true mutants by yellow points (red and green added) as they appear at the same size in both channels. The size of the cleavage product (the sizing ladder can be seen at the left and middle of the image) indicates approximately where the single nucleotide polymorphism is located in the fragment.