Table 3

Tilled genes and mutation density in Caméor mutant population

Tilled genes

Amplicon size (bp)

% of GC in exons

Identified mutants

Screened M2 families

Mutation frequency


Ps CONSTANS-like a (PsCOLa)

1,012

46.30%

11

1,536

1/141 Kb

LectineA

971

40.80%

13

1,536

1/115 Kb

Sucrose transporter (SUT1)

1,014

52.40%

12

1,536

1/130 Kb

Cell wall invertase (cwINV)

1,612

41.50%

12

1,536

1/206 Kb

Serine-threonine proteine kinase (Sym29)

2,457

44.00%

8

768

1/236 Kb

Phosphoenolpyruvate carboxylase (PepC)

1,009

44.40%

25

3,072

1/124 Kb

Lec1-like (L1L)

870

39.10%

21

4,608

1/191 Kb

DOF transcription factor 2 (PsDOF2)

1,200

36.60%

9

3,072

1/410 Kb

Trypsine inhibitor (TI1)

712

34.20%

13

3,840

1/210 Kb

Pea albumine (PA2)

746

38.50%

9

3,072

1/255 Kb

Anther specific protein (End1)

851

40.50%

31

3,072

1/84 Kb

MADS box gene (PM10)

1,302

34.60%

20

4,608

1/300 Kb

MADS box gene (PM2)

1,390

31.30%

28

4,608

1/229 Kb

Tendril-less transcription factor (TL)

1,104

34.00%

28

3,072

1/121 Kb

Eukaryotic translation initiation factor (eiF4e)

1,383

36.90%

36

4,608

1/177 Kb

Eukaryotic translation initiation factor (eIF(iso)4e)

772

36.70%

10

4,608

1/356 Kb

Methyl transferase 1 (Met1)

3,842

40.20%

96

4,704

1/188 Kb

Retinoblastoma related (RBR)

2,959

40.80%

72

4,608

1/112 Kb

Late embryogenesis abundant protein (PsLEAM)

952

44.00%

17

4,608

1/258 Kb

Heat shock protein 22 (HSP22)

622

45.66%

18

4,608

1/159 Kb

Total/mean

26,780

40.12%

467

-

1/200 Kb


Part or all of the Caméor mutant population was screened for mutations in the genes listed. The size of the screened amplicon, the number of mutants identified and the mutation frequency for each amplicon are indicated. The average mutation frequency was estimated to one mutation per 200 kb and is calculated as in Greene et al. [6], except that we have summed the sizes of all the amplicons and we divided by the total number of identified mutants.

Dalmais et al. Genome Biology 2008 9:R43   doi:10.1186/gb-2008-9-2-r43

Open Data