|
Tilled genes and mutation density in Caméor mutant population |
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| Tilled genes |
Amplicon size (bp) |
% of GC in exons |
Identified mutants |
Screened M2 families |
Mutation frequency |
|
|
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| Ps CONSTANS-like a (PsCOLa) |
1,012 |
46.30% |
11 |
1,536 |
1/141 Kb |
| LectineA |
971 |
40.80% |
13 |
1,536 |
1/115 Kb |
| Sucrose transporter (SUT1) |
1,014 |
52.40% |
12 |
1,536 |
1/130 Kb |
| Cell wall invertase (cwINV) |
1,612 |
41.50% |
12 |
1,536 |
1/206 Kb |
| Serine-threonine proteine kinase (Sym29) |
2,457 |
44.00% |
8 |
768 |
1/236 Kb |
| Phosphoenolpyruvate carboxylase (PepC) |
1,009 |
44.40% |
25 |
3,072 |
1/124 Kb |
| Lec1-like (L1L) |
870 |
39.10% |
21 |
4,608 |
1/191 Kb |
| DOF transcription factor 2 (PsDOF2) |
1,200 |
36.60% |
9 |
3,072 |
1/410 Kb |
| Trypsine inhibitor (TI1) |
712 |
34.20% |
13 |
3,840 |
1/210 Kb |
| Pea albumine (PA2) |
746 |
38.50% |
9 |
3,072 |
1/255 Kb |
| Anther specific protein (End1) |
851 |
40.50% |
31 |
3,072 |
1/84 Kb |
| MADS box gene (PM10) |
1,302 |
34.60% |
20 |
4,608 |
1/300 Kb |
| MADS box gene (PM2) |
1,390 |
31.30% |
28 |
4,608 |
1/229 Kb |
| Tendril-less transcription factor (TL) |
1,104 |
34.00% |
28 |
3,072 |
1/121 Kb |
| Eukaryotic translation initiation factor (eiF4e) |
1,383 |
36.90% |
36 |
4,608 |
1/177 Kb |
| Eukaryotic translation initiation factor (eIF(iso)4e) |
772 |
36.70% |
10 |
4,608 |
1/356 Kb |
| Methyl transferase 1 (Met1) |
3,842 |
40.20% |
96 |
4,704 |
1/188 Kb |
| Retinoblastoma related (RBR) |
2,959 |
40.80% |
72 |
4,608 |
1/112 Kb |
| Late embryogenesis abundant protein (PsLEAM) |
952 |
44.00% |
17 |
4,608 |
1/258 Kb |
| Heat shock protein 22 (HSP22) |
622 |
45.66% |
18 |
4,608 |
1/159 Kb |
| Total/mean |
26,780 |
40.12% |
467 |
- |
1/200 Kb |
|
Part or all of the Caméor mutant population was screened for mutations in the genes listed. The size of the screened amplicon, the number of mutants identified and the mutation frequency for each amplicon are indicated. The average mutation frequency was estimated to one mutation per 200 kb and is calculated as in Greene et al. [6], except that we have summed the sizes of all the amplicons and we divided by the total number of identified mutants. | |||||
Dalmais et al. Genome Biology 2008 9:R43 doi:10.1186/gb-2008-9-2-r43 |
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