Log on / register
BioMed Central home | Journals A-Z | Feedback | Support | My details
.refereed research
 |  |  |  |  | 


Open AccessMethod

Construction, alignment and analysis of twelve framework physical maps that represent the ten genome types of the genus Oryza

HyeRan Kim1 email, Bonnie Hurwitz2 email, Yeisoo Yu1 email, Kristi Collura1 email, Navdeep Gill3 email, Phillip SanMiguel4 email, James C Mullikin5 email, Christopher Maher6 email, William Nelson7 email, Marina Wissotski1 email, Michele Braidotti1 email, David Kudrna1 email, José Luis Goicoechea1 email, Lincoln Stein2 email, Doreen Ware2,8 email, Scott A Jackson3 email, Carol Soderlund7 email and Rod A Wing1 email

1Arizona Genomics Institute, Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721, USA

2Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA

3Department of Agronomy, Purdue University, West Lafayette, Indiana 47907, USA

4Department of Horticulture, Purdue University, West Lafayette, Indiana 47907, USA

5Genome Technology Branch, NHGRI, National Institutes of Health, Bethesda, Maryland, 20892, USA

6Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794, USA

7Arizona Genomics Computational Laboratory, University of Arizona, Tucson, Arizona 85721, USA

8USDA-ARS NAA Plant, Soil and Nutrition Laboratory Research Unit, Ithaca, New York 14853, USA

author email corresponding author email

Genome Biology 2008, 9:R45doi:10.1186/gb-2008-9-2-r45

Published: 28 February 2008

Subject areas: Evolution, Genetics, Genome studies, Plant biology

Abstract

We describe the establishment and analysis of a genus-wide comparative framework composed of 12 bacterial artificial chromosome fingerprint and end-sequenced physical maps representing the 10 genome types of Oryza aligned to the O. sativa ssp. japonica reference genome sequence. Over 932 Mb of end sequence was analyzed for repeats, simple sequence repeats, miRNA and single nucleotide variations, providing the most extensive analysis of Oryza sequence to date.


© 1999-2009 BioMed Central Ltd unless otherwise stated. Part of Springer Science+Business Media.