Figure 5.

Influence of the gene structure and number of expressed transcripts in a comparative splicing analysis between different genes (synthetic data). (a) Estimation of the relative transcript concentrations for different genes. The y-axis shows the MAE (%) between simulated and estimated relative concentration of transcripts. The x-axis shows the different genes used in the simulation (CASP2, HNRPA2B1, BCL2L1, BIRC5, TERT, VEGF, BAX and WT1). The structure of the different transcripts of these genes and the location of probes is shown in Additional file 1 (Figures S1-S8). (b) Prediction of the splicing structure for different genes. The y-axis shows the Hamming distance error rate between real and predicted pre-mRNA splicing structures. (c) Sensitivity of SPACE for different genes. (d) Specificity of SPACE for different genes.