Use of dual-color FISH to validate a BT474 genomic breakpoint. End sequences from clone CHORI518_014-E04 were mapped to chromosomes 1 and 4. Clones RP11-692N22 and RP11-1095F2 were selected from the human RPCI11 library because their sequences map to just outside of tumor bacterial artificial chromosome (BAC) end sequence (BES) locations. These BACs were labeled with fluorescein and Texas red, respectively. Top: two chromosomes containing a merged yellow signal indicating juxtaposition of both probes are indicated with white arrows (and labeled A and B). Bottom: each labeled chromosome is shown with corresponding inverted-DAPI banded chromosome, and red and green image layers. Black arrows identify the region where the red and green probes are juxtaposed to one another. FISH, fluorescence in situ hybridization.
Raphael et al. Genome Biology 2008 9:R59 doi:10.1186/gb-2008-9-3-r59