Table 1

dNGLUC is secreted in the absence of a signal peptide

% secreted


GLUC

96.7 ± 27.6

dNGLUC

30.5 ± 7.8

Actin-dNGLUC

1.5 ± 0.4


GLUC activity was determined in SN and whole cell lysate of 293ET cells transfected with the indicated constructs. The percentage of secreted Gaussia luciferase activity was calculated from three independent transfections.

Ketteler et al. Genome Biology 2008 9:R64   doi:10.1186/gb-2008-9-4-r64

Open Data