Table 2

Genes that induce release of dNGLUC activity in SN

Gene
Fold ratio SN/cells

ASPH
14.2 ± 5.8
BAK1
12.5 ± 2.3
PIR121
12.1 ± 2.7
PERP
10.4 ± 2.5
TBC1D10A
5.5 ± 2.7
FADD
4.9 ± 1.8
BAD
4.8 ± 2.7
CASP8
3.2 ± 1.1

Cells were transfected in 96-well plates with DEVDG2F and cDNA expression vectors from the Origene Trueclone™ collection in triplicates. Activity of GLUC was measured in SN and cell lysates after 26 h and ratios of SN/cellular activity were calculated for each plate. Three wells on each plate were transfected with reporter only to determine the background activity. We show the fold ratio of SN/cellular activity over background averaged from three plates.

Ketteler et al. Genome Biology 2008 9:R64   doi:10.1186/gb-2008-9-4-r64