Table 2

Genes that induce release of dNGLUC activity in SN

Gene

Fold ratio SN/cells


ASPH

14.2 ± 5.8

BAK1

12.5 ± 2.3

PIR121

12.1 ± 2.7

PERP

10.4 ± 2.5

TBC1D10A

5.5 ± 2.7

FADD

4.9 ± 1.8

BAD

4.8 ± 2.7

CASP8

3.2 ± 1.1


Cells were transfected in 96-well plates with DEVDG2F and cDNA expression vectors from the Origene Trueclone™ collection in triplicates. Activity of GLUC was measured in SN and cell lysates after 26 h and ratios of SN/cellular activity were calculated for each plate. Three wells on each plate were transfected with reporter only to determine the background activity. We show the fold ratio of SN/cellular activity over background averaged from three plates.

Ketteler et al. Genome Biology 2008 9:R64   doi:10.1186/gb-2008-9-4-r64

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