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Membrane transporters and protein traffic networks differentially affecting metal tolerance: a genomic phenotyping study in yeast

Roberta Ruotolo, Gessica Marchini and Simone Ottonello*

Author Affiliations

Department of Biochemistry and Molecular Biology, Viale G.P. Usberti 23/A, University of Parma, I-43100 Parma, Italy

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Genome Biology 2008, 9:R67  doi:10.1186/gb-2008-9-4-r67

Published: 7 April 2008

Additional files

Additional data file 1:

Presented is a composite figure showing representative examples of genomic phenotyping screens and related serial dilution assays utilized to assess metal resistance as well as the severity of metal sensitivity phenotypes.

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Additional data file 2:

This file reports the name, phenotype and 'scoring rate', description, cadmium stress microarray and nickel ionome data when available, best hit, human ortholog and related disease (if applicable), as well as the GO ID, of metal tolerance-modulating genes.

Format: XLS Size: 239KB Download file

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Additional data file 3:

This figure shows the interaction subnetworks among gene products whose disruption causes nickel-specific sensitivity.

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Additional data file 4:

This figure shows the interaction subnetworks among gene products whose disruption causes cadmium-specific sensitivity.

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Open Data

Additional data file 5:

This figure documents the altered cadmium tolerance of the fet4Δ, smf1Δ, and rox1Δ mutant strains.

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Additional data file 6:

This figure documents the altered nickel tolerance of the fur4Δ and tna1Δ mutant strains.

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Additional data file 7:

this document reports the metal sensitivity or resistance phenotypes of the 388 mutant strains with an altered cadmium/nickel tolerance, exposed to sublethal concentrations of mercury, arsenite, cobalt, zinc, and iron.

Format: XLS Size: 54KB Download file

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Open Data