Table 1

Estrogen-regulated probe sets that are differentially expressed in wounds from elderly compared to young subjects
Affy ID
 Gene*
 Gene (description)
 Function
 q-value†
 FC‡
Down-regulated probe sets (65)




     207720_at
 LOR
 loricrin
 Major cornified envelope protein
 0
 -235
     201909_at
 RPS4Y1
 ribosomal protein S4, Y-linked 1
 40S ribosomal component
 0
 -142
     215704_at
 FLG
 filaggrin
 Cornified envelope-keratin linker
 9.4E-13
 -114
     206177_s_at
 ARG1
 arginase, liver
 Delayed healing-associated
 9.2E-11
 -82.0
     206643_at
 HAL
 histidine ammonia-lyase
 Histidine catabolism
 1.5E-10
 -59.0
     206421_s_at
 SERPINB7
 serpin peptidase inhibitor, clade B (ovalbumin), member 7
 Proteinase inhibitor for plasmin
 5.9E-13
 -47.6
     206192_at
 CDSN
 Corneodesmosin
 Desquamation/adhesion
 2.5E-06
 -30.1
     213796_at
 SPRR1A
 small proline-rich protein 1A
 Cornified envelope precursor protein
 1.5E-05
 -29.4
     207324_s_at
 DSC1
 desmocollin 1
 Desmosomal cadherin/adhesion
 9.6E-06
 -28.9
     209719_x_at
 SERPINB3
 serpin peptidase inhibitor, clade B (ovalbumin), member 3
 Inflammation and cancer-associated
 1.6E-05
 -22.4
     217496_s_at
 IDE
 insulin-degrading enzyme
 Wound fluid/resolution of insulin response
 4.0E-06
 -20.5
     211597_s_at
 HOP
 homeodomain-only protein
 Serum response factor binding
 1.7E-04
 -19.7
     211726_s_at
 FMO2
 flavin containing monooxygenase 2 (non-functional)
 Non-functional oxidative enzyme
 7.0E-04
 -18.9
     220414_at
 CALML5
 calmodulin-like 5
 Epidermal-associated calcium-binding
 2.0E-05
 -17.7
     203328_x_at
 IDE
 insulin-degrading enzyme
 Wound fluid/resolution of insulin response
 1.4E-05
 -17.4
     210413_x_at
 SERPINB4
 serpin peptidase inhibitor, clade B (ovalbumin), member 4
 Cancer and inflammation-associated
 3.1E-05
 -15.8
     219795_at
 SLC6A14
 solute carrier family 6 (amino acid transporter), member 14
 Amino acid transport/obesity
 6.9E-04
 -15.6
     210074_at
 CTSL2
 cathepsin L2
 Lysosomal cysteine proteinase
 3.8E-05
 -15.5
     222242_s_at
 KLK5
 kallikrein 5
 Desquamation, angiogenesis and cancer
 4.0E-05
 -15.0
     201348_at
 GPX3
 glutathione peroxidase 3 (plasma)
 Protection from oxidative damage
 1.2E-05
 -14.8
     202018_s_at
 LTF
 lactotransferrin
 Inflammatory-cell-derived antioxidant
 4.6E-02
 -14.5
     205185_at
 SPINK5
 serine peptidase inhibitor, Kazal type 5
 Anti-inflammatory/anti-microbial
 3.8E-05
 -14.4
     211906_s_at
 SERPINB4
 serpin peptidase inhibitor, clade B (ovalbumin), member 4
 Cancer and inflammation-associated
 5.7E-05
 -12.4
     219232_s_at
 EGLN3
 egl nine homolog 3 (C. elegans)
 Hypoxia-inducible apoptosis-inducing
 1.4E-05
 -12.1
     213256_at
 MARCH3
 membrane-associated ring finger (C3HC4) 3
 Poorly characterized ubiquitin ligase
 1.6E-05
 -12.1
     204733_at
 KLK6
 kallikrein 6 (neurosin, zyme)
 Hormone regulated serine protease
 1.4E-05
 -11.9
     202179_at
 BLMH
 bleomycin hydrolase
 Cysteine peptidase
 2.1E-03
 -11.8
     214549_x_at
 SPRR1A
 small proline-rich protein 1A
 Cornified envelope precursor protein
 1.6E-04
 -11.3
     207908_at
 KRT2
 keratin 2 (epidermal ichthyosis bullosa of Siemens)
 Supra-basally expressed cytokeratin
 1.2E-03
 -11.1
     210338_s_at
 HSPA8
 heat shock 70 kDa protein 8
 ERalpha-inhibiting heat shock protein
 9.9E-04
 -10.6
     209720_s_at
 SERPINB3
 serpin peptidase inhibitor, clade B (ovalbumin), member 3
 Inflammation and cancer-associated
 3.3E-04
 -10.5
     201849_at
 BNIP3
 BCL2/adenovirus E1B 19 kDa interacting protein 3
 Mitochondrial apoptosis-inducing
 2.7E-04
 -10.1
     205916_at
 S100A7
 S100 calcium binding protein A7
 Chemotactic psoriasis-associated protein
 1.7E-04
 -10.0
     204952_at
 LYPD3
 LY6/PLAUR domain containing 3
 Upregulated in migrating keratinocytes
 1.2E-03
 -9.7
     206595_at
 CST6
 cystatin E/M
 Cysteine protease inhibitor
 1.7E-06
 -9.3
     203327_at
 IDE
 insulin-degrading enzyme
 Wound fluid/resolution of insulin response
 7.0E-04
 -9.3
     209555_s_at
 CD36
 CD36 molecule
 Thrombospondin receptor
 4.0E-03
 -9.2
     219532_at
 ELOVL4
 elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
 Skin barrier-promoting fatty acid elongase
 1.5E-05
 -9.2
     209126_x_at
 KRT6B
 keratin 6B
 Injury-associated keratin
 1.7E-03
 -9.1
     212573_at
 ENDOD1
 endonuclease domain containing 1
 Unknown
 8.3E-04
 -9.0
     214599_at
 IVL
 involucrin
 Early cornified envelope protein
 2.8E-03
 -8.8
     209218_at
 SQLE
 squalene epoxidase
 Rate-limiting sterol biosynthesis enzyme
 7.2E-04
 -8.8
     207356_at
 DEFB4
 defensin, beta 4
 Antimicrobial peptide
 6.0E-03
 -8.8
     210138_at
 RGS20
 regulator of G-protein signaling 20
 GTPase-activating protein
 8.1E-04
 -8.7
     202504_at
 TRIM29
 tripartite motif-containing 29
 Cancer-associated transcription factor
 2.2E-03
 -8.6
     205016_at
 TGFA
 transforming growth factor, alpha
 IFN-induced/epidermal regeneration
 1.0E-03
 -8.5
     209309_at
 AZGP1
 alpha-2-glycoprotein 1, zinc
 TNFA-regulated prostate-cancer marker
 3.5E-04
 -8.5
     209800_at
 KRT16
 keratin 16 (focal non-epidermolytic palmoplantar keratoderma)
 Hyperproliferation and healing-associated keratin
 1.2E-03
 -8.3
     205778_at
 KLK7
 kallikrein 7 (chymotryptic, stratum corneum)
 Innate immunity/desquamation
 1.2E-05
 -8.3
     219756_s_at
 POF1B
 premature ovarian failure, 1B
 Unknown
 3.9E-05
 -8.1
     214091_s_at
 GPX3
 glutathione peroxidase 3 (plasma)
 Protection from oxidative damage
 3.0E-03
 -8.1
     203585_at
 ZNF185
 zinc finger protein 185 (LIM domain)
 Actin-associated tumor suppressor
 1.4E-03
 -8.1
     206008_at
 TGM1
 transglutaminase 1
 CE formation/epidermal differentiation
 4.6E-05
 -8.0
     202037_s_at
 SFRP1
 secreted frizzled-related protein 1
 Repressor of WNT signaling
 6.6E-04
 -7.9
     202539_s_at
 HMGCR
 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
 Rate-limiting cholesterol synthesis enzyme
 7.4E-04
 -7.8
     203575_at
 CSNK2A2
 casein kinase 2, alpha prime polypeptide
 p53 phosphorylation, WNT signaling
 4.6E-04
 -7.7
     206884_s_at
 SCEL
 sciellin
 Cornified envelope precursor protein
 2.1E-04
 -7.5
     204284_at
 PPP1R3C
 protein phosphatase 1, regulatory (inhibitor) subunit 3C
 Regulates a wide variety of cellular functions
 9.9E-04
 -7.4
     266_s_at
 CD24
 CD24 molecule
 Marker for epithelial neoplasms
 2.7E-04
 -7.4
     203914_x_at
 HPGD
 hydroxyprostaglandin dehydrogenase 15-(NAD)
 Main enzyme for prostaglandin degradation
 1.6E-04
 -7.3
     219410_at
 TMEM45A
 transmembrane protein 45A
 Hox-regulated/reproductive tissue expressed
 8.1E-04
 -7.3
     206488_s_at
 CD36
 CD36 molecule
 Thrombospondin receptor
 1.2E-05
 -7.3
     204881_s_at
 UGCG
 UDP-glucose ceramide glucosyltransferase
 Keratinocyte glucosyltransferase
 1.8E-03
 -7.1
     213933_at
 PTGER3
 prostaglandin E receptor 3 (subtype EP3)
 Impaired wound healing in null mouse
 8.3E-04
 -7.1
     216379_x_at
 CD24
 CD24 molecule
 Marker for epithelial neoplasms
 7.9E-04
 -7.0






Up-regulated probe sets (8)




     221728_x_at
 XIST
 X (inactive)-specific transcript
 X chromosome inactivation
 2.4E-12
 191.8
     214218_s_at
 XIST
 X (inactive)-specific transcript
 X chromosome inactivation
 1.0E-09
 56.2
     206211_at
 SELE
 selectin E (endothelial adhesion molecule 1)
 Endothelial-leukocyte adhesion
 9.0E-02
 8.5
     211600_at
 PTPRO
 protein tyrosine phosphatase, receptor type, O
 New marker of podocyte injury
 5.0E-04
 8.4
     220940_at
 KIAA1641
 KIAA1641
 Unknown
 1.0E-04
 8.3
     203915_at
 CXCL9
 chemokine (C-X-C motif) ligand 9
 Interferon induced, TH1 response
 6.3E-02
 7.3
     204324_s_at
 GOLPH4
 golgi phosphoprotein 4
 Protein export
 8.3E-04
 7.3
     201205_at
 RRBP1
 ribosome binding protein 1 homolog 180 kDa (dog)
 Developmentally regulated extracellular matrix glycoprotein
 6.3E-03
 7.3

*Genes in bold have been validated by qPCR. †CyberT-derived multiple testing corrected q-value. ‡Fold change (old/young).

Hardman and Ashcroft Genome Biology 2008 9:R80   doi:10.1186/gb-2008-9-5-r80

Open Data