Additional data file 2.
Presented is a figure showing the analysis of GIF expression in platypus and echidna tissues. Total RNA from platypus and echidna (T. aculeatus) tissues was subjected to RT-PCR using specific primers for GIF and GAPDH as control. The amplification products were separated in a 3% agarose gel, showing the highest expression of GIF in echidna pancreas, as well as in liver from platypus an echidna, whereas no expression could be detected in platypus brain or muscle. The identity of echidna GIF was confirmed by direct nucleotide sequencing of the amplified product.
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Ordoñez et al. Genome Biology 2008 9:R81 doi:10.1186/gb-2008-9-5-r81