Figure 2.

Nested RT-PCR for the detection of the TMPRSS2:ERG fusion. Samples from the microarray data set, where sufficient material was available, were subjected to nested RT-PCR to detect the presence of the TMPRSS2:ERG fusion product. The fusion product was detected in 6 of 10 samples and undetectable in the remainder (samples marked ND). cDNA from the fusion positive cell line VCaP was used as a positive control, water was substituted in place of cDNA for the negative control.