Figure 3.

Validation of selected genes by quantitative real time PCR. (a) RT-PCR confirmation of Affymetrix array data on genes associated with prostate cancer (all changes in expression were significant at p < 0.05). Changes between stem and committed cells are indicated in blue, while malignant versus benign changes are indicated in red. (b) Validation of average changes in gene expression between stem and committed basal populations detected by Affymetrix array (red bars) and RT-PCR techniques (blue bars). (c) Validation of average changes in gene expression between malignant and benign stem cell populations detected by Affymetrix (red bars) and RT-PCR techniques (blue bars).