Figure 4.

Validation of selected genes by flow cytometry and immunocytochemistry. (a) Flow cytometry analysis of prostate cancer cells co-stained with antibodies to CD133 and the NF-κB p65 subunit. (b) Confocal image of sorted CD133⁺ cancer cells stained with an antibody to the NF-κB p65 subunit (green) counterstained with DAPI (blue). Nuclear concurrence of two signals is indicated by a cyan colour. (c-e) Flow cytometry analysis of prostate cancer cells co-stained with antibodies to CD133 and ZO1/TJP1 (c) or ZO2/TJP2 (d) or PAPPA (e).